Table 1.
Isoforms tested, marker reactions, incubation conditions and Km used in the inhibition study.
| CYPs | Marker reactions | Substrate concentration (μM) | Protein concentration (mg/mL) | Incubation time (min) | Estimated Km (μM) |
|---|---|---|---|---|---|
| 1A2 | Phenacetin O-deethylation | 40 | 0.2 | 30 | 48 |
| 3A4 | Testosterone 6β-hydroxylation | 50 | 0.5 | 10 | 53 |
| 2A6 | Coumarin 7-hydroxylation | 1.0 | 0.1 | 10 | 1.5 |
| 2E1 | Chlorzoxazone 6-hydroxylation | 120 | 0.4 | 30 | 126 |
| 2D6 | Dextromethorphan O-demethylation | 25 | 0.25 | 20 | 4.8 |
| 2C9 | Diclofenac 4'-hydroxylation | 10 | 0.3 | 10 | 13 |
| 2C19 | S-Mephenytoin 4-hydroxylation | 100 | 0.2 | 40 | 105 |
| 2C8 | Paclitaxel 6α-hydroxylation | 10 | 0.5 | 30 | 16 |