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. 2018 Nov 30;22(6):429–437. doi: 10.1080/19768354.2018.1549099

Figure 4.

Figure 4.

The up-regulation and nuclear translocation of Nrf2 by GAS. (A, B) C6 cells were treated with 100 μM of GAS for 3, 6, 9, or 12 h and total (A), nuclear (B) and cytoplasmic Nrf2 levels (C) were determined by immunoblotting. (D) Double fluorescent staining was performed using anti-Nrf2 antibody and DAPI after treating C6 cells with GAS (100 uM) for 3, 6, 9, or 12 h. Arrowheads indicate Nrf2 translocation from cytoplasm to nucleus and arrows indicate cytoplasmic localization of Nrf2. (D) C6 cells were treated with 100 μM GAS for 3, 6, 9, or 12 h and protein levels of HO-1, GCLM, and α-tubulin were determined by immunoblotting. The scale bar represents 100 μm. Results are presented as means ± SEMs (n = 4) **p < 0.01 versus treatment naïve controls.