Figure 2.

miR-124 Down-Regulates Fatty Acid Oxidation

(A) Relative mRNA expression levels of miR-124 predicted targets with roles in fatty acid degradation in Huh7.5 cells transfected with control (Con-miR) or miR-124 mimic, as measured by qRT-PCR (n > 5).

(B) Immunoblot analysis of protein expression levels of ECI2 and HADHA (lower band) in miR-124- or control mimic- (Con-miR) transfected Huh7.5 cells. Three independent biological replicates are shown, and total protein detection serves as the loading control. The blot is cropped to emphasize relevant bands.

(C) Relative mRNA expression levels of miR-124 predicted targets with roles in carnitine biosynthesis (n > 6).

Data are represented as the mean ± SEM (*p < 0.05). See also Tables S1 and S2.