Figure 4.

miR-124 Down-Regulates Genes associated with Triglyceride Hydrolysis

(A) Relative mRNA expression levels of PNPLA2, CES1, NR1H4, and AADAC in control and miR-124-mimic-transfected cells as assessed by qRT-PCR analysis (n > 5). Data are represented as the mean ± SEM (*p < 0.05).

(B) Immunoblot analysis of protein expression levels of AADAC in miR-124- or control mimic- (Con-miR) transfected Huh7.5 cells. Three independent biological replicates are shown, and total protein detection serves as loading control. Blot is cropped to emphasize relevant bands.