Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 7;66(10):2126–2136. doi: 10.1002/glia.23466

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors. Glia published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Total internal reflection microscopy (TIRF) imaging of lysosomal vesicle polyP release. Representative images (a) and traces (measurement of polyP in individual vesicles) (A1) depicting partial release of polyP from lysosomes upon application of calcium ionophore ionomycin (1 μM). Partial lysosomal polyP release upon acidification of the cytosol with NH4Cl (b,b1). (c,c1) Application of glycyl‐l‐phenylalanine‐β‐naphthylamide (GPN, 100 μM) results in the collapse of lysosomes and release of polyP to the cytoplasm (see increase of DAPI‐polyP fluorescence (blue) in cytosol). (d) Summary data showing release of polyP from the lysosomes upon different stimuli [Color figure can be viewed at http://wileyonlinelibrary.com]