Table 2.
PCR and virus isolation results
| Sample name | Sample type | Virus isolated | PCR/qRT‐PCRa | Nested PCR | Partial N sequence | Full‐length sequence |
|---|---|---|---|---|---|---|
| Bangladesh/B170/Bhola/2012 | Lung | + | + | + | ||
| Bangladesh/B167/Sylhet/2013 | Nasal Swab | + | + | |||
| Bangladesh/B174/Bhola/2013 | Lung | + | + | + | ||
| Bangladesh/B147/Gangi/2013 | Nasal Swab | + | + | |||
| Bangladesh/B70/Sirajgonj/2014 | Nasal Swab | + | + | |||
| Bangladesh/B53/Savar/2015 | Milk | + | +/30.1 | + | ||
| Bangladesh/B54/Savar/2015 | Lung | + | + | |||
| Bangladesh/B40/Chuadanga/2015 | Nasal Swab | + | + | |||
| Bangladesh/B51/Chuadanga/2015 | Faeces | + | + | |||
| Bangladesh/B52/Chuadanga/2015 | Milk | +/24.76 | + | |||
| Bangladesh/B27/Munsigonj/2015 | Nasal Swab | + | + | |||
| Bangladesh/B2/Nihkanchari/2015 | Nasal Swab | + | + | |||
| Bangladesh/B18/Nihkanchari/2015 | Milk | + | +/24.61 | + | ||
| Bangladesh/B19/Nihkanchari/2015 | Milk | + | +/23.54 | + | ||
| Bangladesh/B20/Nihkanchari/2015 | Faeces | + | ||||
| Bangladesh/B21/Nihkanchari/2015 | Faeces | |||||
| Bangladesh/B22/Nihkanchari/2015 | Faeces | + | ||||
| Bangladesh/B23/Nihkanchari/2015 | Faeces | + | ||||
| Bangladesh/B24/Nihkanchari/2015 | Faeces |
Note. The qRT‐PCR results are presented as cycle threshold (Ct) values where applicable. The empty cells indicate negative result. aqRT‐PCR was carried out only on milk samples.