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. 2018 Sep 17;234(2):1522–1533. doi: 10.1002/jcp.27017

Figure 2.

Figure 2

MiR‐24‐3p promotes cell cycle progression from G0/G1 to the S phase in mammary epithelial cells. MAC‐T cells were exposed to the miR‐24‐3p mimics (mimics, a) and/or miR‐24‐3p inhibitor (inhibitor, b), as well as their corresponding negative controls (mimics NC and/or inhibitor NC), for 24 hr and assessed for the distribution of cell cycle phases after propidium iodide (PI) staining. The percentages of cells in G0/G1, S, and G2/M phases are shown in (a,b), respectively. Representative FACS images of cell phase analyses illustrated changes of cell cycle in MAC‐T cells upon miR‐24‐3p overexpression (a) and/or low‐expression (b), compared with the negative controls. The cell cycle phase distribution (%) is indicated within each panel. The gene expression of cell cycle regulators specific to G1/S phase, such as cyclinD1, CDK4, CDK6, p18, and p27, were detected in the same miR‐24‐3p mimics (c) and or inhibitor (d) transfected MAC‐T cells using quantitative RT‐PCR. All of the experiments were performed three times for each transfection. **p < 0.01 and *p < 0.05. MAC‐T: mammary epithelial cell; NC: negative control; RT‐PCR: reverse transcription polymerase chain reaction [Color figure can be viewed at wileyonlinelibrary.com]