Table 2.
Mean concentrations, coefficient of variation (CV), and mean accuracy of measured steroid hormones in walrus bone tissue spiked with each hormone's respective concentrations along a standard calibration curve (i.e., “Concentration (ng)”). Respective concentrations for each hormone have n = 5 replicates. Accuracy was determined following Zhang et al45
| Hormone | Concentration (ng) | Mean concentration (ng) | CV (%) | Mean accuracy (%) |
|---|---|---|---|---|
| Progesterone | 3200 | 3206.04 | 9.33 | 100.19 |
| 1600 | 1535.04 | 0.98 | 95.94 | |
| 800 | 869.73 | 6.50 | 108.72 | |
| 400 | 445.19 | 6.69 | 111.30 | |
| 200 | 199.90 | 6.11 | 99.95 | |
| 100 | 102.58 | 5.70 | 102.58 | |
| 50 | 50.13 | 7.67 | 100.25 | |
| 0.5 | 0.53 | 3.39 | 105.54 | |
| Testosterone | 500 | 499.76 | 1.84 | 99.95 |
| 250 | 251.30 | 4.17 | 100.52 | |
| 125 | 124.73 | 2.19 | 99.78 | |
| 75 | 71.92 | 1.61 | 95.92 | |
| 0.5 | 0.54 | 3.15 | 107.07 | |
| Cortisol | 500 | 498.09 | 4.03 | 99.62 |
| 250 | 255.25 | 2.99 | 102.10 | |
| 125 | 125.19 | 1.10 | 100.15 | |
| 75 | 69.65 | 3.14 | 92.87 | |
| 0.5 | 0.54 | 8.30 | 107.50 | |
| Estradiol | 200 | 200.90 | 2.64 | 100.45 |
| 100 | 100.22 | 3.54 | 100.22 | |
| 50 | 48.99 | 7.96 | 97.98 | |
| 25 | 25.24 | 3.64 | 100.97 | |
| 5 | 5.40 | 8.56 | 107.99 |