Table 4.
Binding kinetics of PfHsp70‐x and human hop
| Ligand | Analyte | k a (1/Ms) | k d (1/s) | K D (M) | X 2 |
|---|---|---|---|---|---|
| PfHsp70‐x | hHop | 4.30 (± 0.40) e3 | 2.30 (± 0.35) e−5 | 3.01 (± 0.15) e−8 | 1.1 |
| hHop +ADP | 3.30 (± 0.12) e3 | 3.70 (± 0.43) e−5 | 2.31 (± 0.25) e−8 | 1.31 | |
| hHop +ATP | 3.47 (± 0.40) e3 | 4.40 (± 0.36) e−4 | 3.80 (± 0.35) e−7 | 1.5 | |
| PfHsp70‐xT | hHop | 2.47 (± 0.14) e1 | 1.01 (± 0.22) e−5 | 2.10 (± 0.12) e−6 | 3.92 |
| hHop+ADP | 4.26 (± 0.54) e1 | 2.10 (± 0.24) e−5 | 1.66 (± 0.05) e−6 | 1.09 | |
| hHop +ATP | 5.41 (± 0.10) e1 | 3.20 (± 0.14) e−4 | 4.10 (± 0.13) e−5 | 3.2 |
Table legends: k a is the association rate constant, k d dissociation rate constant, K D equilibrium constant, X 2 is the chi‐squared value correlation for SPR sensorgram fitting to the Langmuir model. Data is presented as mean plus/minus SD.