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EtOH or imiquimod exposure increases the level of let‐7b in microvesicles released from primary human hepatocytes. tRNA was isolated from hepatocyte‐derived MVs as described in Materials and Methods. 5 ng of MV‐associated tRNA was used for qPCR for the TLR7‐binding microRNAs miR‐21 and let‐7b in MVs released from hepatocytes exposed to EtOH (A) or imiquimod (B). Data are shown as mean ± SEM (n = 4). a, p < 0.05 compared to control.
