Figure 2.

Kisspeptin enhances GSIS in cultured human donor islets and a human pancreatic β‐cell line. A, Insulin secretion was measured in human islet preparations (n = 6 donors) at low (3 mM) and high (17 mM) concentrations of glucose in the presence of increasing concentrations of kisspeptin (0 nM [blue bars], 2.7 nM [red bars] or 1000 nM [black bars]). Insulin secretion was normalized to percentage of total secretion; * p < 0.05 (Friedman test with Dunn's multiple comparison tests). B, Insulin secretion in cultured EndoC‐βH1 cells (n = 3 experiments) was measured at high glucose concentrations (15 mM) in the presence of increasing amounts of kisspeptin (0 nM [blue bars], 100 nM [green bars] or 1000 nM [black bars]). Insulin secretion was normalized to percentage of total secretion; * p < 0.05 (two‐way ANOVA with Dunnett's multiple comparison tests). Data presented as mean ± SEM