Figure 1.

GR silencing in EPI‐EC and BBB integrity. A, ECs isolated from drug‐resistant epileptic brain resections were transfected (EPI‐EC GR siRNA; n = 6). EPI‐EC non‐siRNA and control human brain microvascular endothelial cells (HBMECs) were compared. Immunocytochemistry shows successful GR silencing by a decrease in GR levels in EPI‐ECs. Decreased PXR, CYP3A4, and MDR1 were observed in EPI‐EC GR siRNA. A1, Quantification of fluorescent signals showed a significant decrease in expression levels of GR (P ≤ 0.01), PXR (P ≤ 0.05), CYP3A4 (P ≤ 0.01), and MDR1 (P ≤ 0.01) in EPI‐EC GR siRNA (mean ± SEM). B, Transendothelial electrical resistance (TEER) measurement showed development of a barrier characterized by an increase in TEER (~600‐800 Ω/cm²) normalized with the baseline TEER reading. No statistical difference was found among TEER readings in EPI‐EC GR siRNA, EPI‐EC non‐siRNA, and HBMEC DIV‐BBB. All experiments were repeated in triplicate. C, The cellular metabolic pattern within the DIV‐BBB setup showed no significant difference in the pattern of glucose and lactate levels over time during the DIV‐BBB formation