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. 2018 Sep 14;48(11):1904–1914. doi: 10.1002/eji.201847699

Figure 3.

Figure 3

FKBP51 knock down increases steroid sensitivity. (A) RNA expression of Gilz (Tsc22d) 16 h after dexamethasone treatment in A549 cells. Cells were transfected with a scrambled (Scr) siRNA (negative control) or a siRNA, knocking down FKBP51 (siFKBP51) Gilz expression was referred to the control and normalized with HPRT. A549 cells were transfected like it is described in the Materials and methods. Data are shown as means ± SD of three independent experiments. One‐way ANOVA, Tukey post‐test; *p < 0.05. (B) Dexamethasone dose response curves. A549 cells were siRNA transfected and stimulated with decreasing amounts of dexamethasone and IL‐1β. Supernatants were used to determine IL‐8 and IL‐6 levels by ELISA. The table indicates the EC50 concentrations of dexamethasone for IL‐8 and IL‐6 reduction in the control‐, the scrambled‐ and the siFKBP51 treated group. Statistics: Fitted curves are means ± SD of three independent experiments. Extra sum‐of‐square F test to test the hypothesis for same EC50 values; EC50s are different between the siFKBP51 group and the controls for IL‐8 and IL‐6 with p < 0.0009.