Figure 4.

Co‐Immunoprecipitation of FKBP51 and Hsp90. (A) Mass spectrometry (MS)‐based analysis of FKBP51 co‐immunoprecipitates. A549 cells were transfected with SBP‐tagged FKBP51. IP‐fraction was used for a Coomassie gel and cropped single bands were used for tryptic based MS analysis (MS analysis was done from one IP (n = 1) and verified by four independent IPs with western blot detection). Listed are a couple of relevant proteins. A detailed table of analyzed peptides and MASCOT Scores is depicted in the Supporting Section. (B) Co‐ IP with FKBP51 and Hsp90. Depicted are representative Western Blots of four independent experiments. “FT” stands for flow through fraction, “IP” for the eluted, precipitated fraction. FT‐ and IP fractions do not contain equal amounts of loaded protein.