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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Aug 21;123:26–37. doi: 10.1016/j.yjmcc.2018.08.015

Figure 4. Simultaneous measurements of myofilament lattice spacing and steady-state isometric force at SL 2.1 μm.

Figure 4.

A) Representative images of CMPs small-angle X-ray diffraction in various pCa solutions. The top horizontal line shows a deviation of the d1,0 as contraction develops and the bottom red line intersects each d1,0 intensity at the center to demonstrate the decrease in lattice spacing that is being reported. Note the reciprocal relationship between d1,0 equatorial distances and lattice spacing. B and C) Relative force vs. lattice spacing. The force values were normalized to the maximum force of each individual CMPs. D and E) Ca2+-dependence of myofilament lattice spacing. Dashed lines were drawn by eye. *p<0.05 vs. Ctrl, statistical significance was determined by Mann-Whitney U test. A minimum of 20 X-ray shots were obtained at each pCa, for exact number of CMPs and shots and refer to supplementary Table 2 Data are shown as mean ± S.E.