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. 2018 Oct 24;16(11):2289–2299. doi: 10.1111/jth.14275

Figure 4.

Figure 4

Characterization of recombinant ADAMTS‐13 expressed in CHO cell lines and purified for in vivo experiments. (A) The proteolytic activities of ADAMTS‐13 expressed in HEK293S and CHO K1 stable cell lines (in conditioned medium) and purified ADAMTS‐13 expressed in CHO K1 cells were determined with the FRETS‐VWF73 assay. Pairwise t‐tests showed no significant difference in the activity of ADAMTS‐13 expressed in the two different cell lines or between purified ADAMTS‐13 and that in conditioned medium. The enhancement of purified ADAMTS‐13 activity by VWF D4‐CK was also determined. The activity of each VWF D4‐CK‐treated sample was compared with the corresponding ADAMTS‐13 activity (**P < 0.01, ****P < 0.001). All results are mean ± standard deviation (SD), n = 3. (B, C) Adult wild‐type (WT) C57bl6 mice were injected with purified ADAMTS‐13 (WT or gain‐of‐function [GoF]) expressed in either HEK293S or CHO K1 cell lines. The plasma ADAMTS‐13 antigen level in these mice was determined at 5, 20 and 40 min after injection. Results are mean ± SD, n = 3–5. VWF, von Willebrand factor.