Table 1.

Detection of Aquavolonida 18S rRNA phylotypes in various habitat types using two different PCR strategies. Dashes indicate that the primer strategy did not amplify Aquavolonida from that habitat type

Habitat type	PCR strategy I – ‘core’ Aquavolonida	PCR strategy II – general Aquavolonida diversity
	Positive samples	Positive samples	Aquavolonida lineage(s) detected
Marine sediment	0 of 32	0 of 32	–
Marine plankton	0 of 32	0 of 32	–
Estuarine gradient	1 of 16a	0 of 16	–
Soils	0 of 16b	11 of 16	NC10‐D (94% of clones) and NC10‐B (6% of clones)
Freshwater sediment	8 of 16	14 of 16	NC10‐A (63% of clones) and NC10‐B (37% of clones)
Freshwater river biofilm	7 of 8	7 of 8	NC10‐A (80% of clones) and NC10‐B (20% of clones)
Freshwater plankton	16 of 16	16 of 16	NC10‐A (67% of clones) and NC10‐B (33% of clones)

^aThe only positive sample was from the freshwater end of the estuarine gradient.

^bOn soil samples this PCR strategy can generate false positives (amplification of lobose amoebae with the primers used in the first PCR).