Table 2.
Sequence signatures observed in the 18S rRNA gene supporting the monophyly of Aquavolonida ord. nov. and its relationship with Tremulida and NC12
| Helix and positionsa | Specific sequence motifb | Consensus sequence in other eukaryotes |
|---|---|---|
| Helix 11, 229‐234 and 248‐252 | [R(A)GCYY]–[RRGC^Y] | [R(A)UCGC]–[GCGA^Y] |
| Between helices 20 & 21, 537‐545 | GCGGUAAUA | GCGGUAAUU |
| Helix 25, 866‐878 and 885‐900 | [UCHDNGG(CCA)GAG]–[UUC(UAUGAU)UCNBKGA] | [UYMRYKG(YCA)GAG]–[UUC(UYGGAU)YYRYKRA] |
| Helix 29, 1016‐1028 and 1041‐1053 | [CGR(GA)UBRGYSVW]–[UBBRYYDG(CA)YCG] | [AGG(GA)UYRGNRRR]–[YYYNYYRG(CA)CCU] |
| Helix 45, 1451‐1456 and 1506‐1511 | [GUUGCD]–[BGCAAC] | [GAYRVR]–[YBYRUC] |
| Helix 48, 1565‐1573 and 1579‐1587 | [AA(C)GCRYKU]–[GMRYGC(A)UU] | [AR(C)GCRAGU]–[RCUYGC(R)YU] |
a
Helix numbering according to Wuyts et al. (2000), and positions given using NC10 environmental clone AY2009C14 (accession number HQ219347) as a reference.
b
Bold and underlined: three complementary and two single‐sequence signatures that together are uniquely found in Aquavolonida ord. nov. and are used as a diagnostic feature of this order; bold: two complementary sequence signatures supporting a clade comprising Aquavolonida, Tremulida, and NC12; underlined: single‐sequence signature shared by this clade and Endomyxa, but not core Cercozoa (=Filosa).