Fig. 6.
PrlR recruits fast STAT5-independent signaling pathways to acutely modify the biophysical properties of cells in the preoptic region. A–C. Current-clamp recordings of MPA neurons unresponsive to prolactin (A), responsive of a female on diestrus (B) and responsive of a lactating N-STAT5 KO female (C). Dashed lines indicate the resting membrane potential. D. Identification of prolactin excited neuron in the MPA for whole-cell patch-clamp recording (low magnification for anatomical reference). E. Bar graphs showing the change in the membrane potential of unresponsive (n = 14) and responsive (female on diestrus, n = 4; lactating female, n = 2) MPA neurons after prolactin application. F. I–V plot from MPA neurons responsive to prolactin (n = 14) illustrating the characteristic reduction in input resistance during the prolactin-induced depolarization (reversal = −31 mV). Abbreviations: 3v, third ventricle. *p < 0.01 compared to unresponsive cells (one-way ANOVA followed by Newman–Keuls test).