Figure 6.

CD74 and CXCR4 on human B cells differentially control proinflammatory gene expression, proliferation, and sensitivity to Fas‐mediated apoptosis. B cells from healthy blood were in vitro‐activated with a‐IgM and subsequently treated with anti‐CD74 antibody (LN2) or AMD3100. Data were compared to their respective controls for relative NF‐κB, IL‐6, and TNF‐α mRNA expression after 24 h using qPCR (A, n = 4–6, measured in duplicates), as well as CFSE‐based proliferation (B and C, n = 5) and Fas (CD95) surface levels (D, n = 5) after 3 days using FACS. Stimulations have been done in three individual experiments with two controls per group per experiment. All used controls were set at 1 (dotted line). Data are shown as mean ± SEM. Paired t‐tests were performed to compare groups. *p < 0.05, **p < 0.01.