Figure 6.

Effects of forskolin (FSK) on tumour necrosis factor (ligand) superfamily, member 11 (RANKL) and tumour necrosis factor receptor superfamily, member 11b (OPG) gene expression levels in cultured human dental follicle cells (HDFCs). The HDFCs from four patients were precultured in MEM Alpha medium (α‐MEM) with or without the presence of osteogenic induction medium for 14 d and thereafter cultured in α‐MEM with FSK (10⁻⁶ M) for the stated time periods. The results are presented as the average of two different experiments performed in duplicate. The expression levels of RANKL (A) and OPG (B), measured by RT‐qPCR, are adjusted by standardization based on the expression levels of glucuronidase, beta (GUSB) and are presented as fold increases relative to the control over time (0, 24, and 48 h). The RANKL/OPG ratios are presented as mean ± SEM (n = 5). (C) Ratios of RANKL/OPG gene expression are presented as ▵▵Ct values.