Skip to main content
. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Methods Mol Biol. 2019;1884:215–229. doi: 10.1007/978-1-4939-8885-3_15

Fig. 3. Bivariate plot strategies for analyzing cell populations from Cryo-DISSECT mass cytometry data of the human colon.

Fig. 3.

Cells were gated as objects with positive staining of Ir intercalator and exceeding a certain ion cloud size. Elemental normalization beads were further excluded in the 155 and 175 channels from cell objects. Within the cell object population, epithelial cells were PCK-positive, while PCK-negative cells represented non-epithelial cells, a population of which was CD45+ leukocytes. Amongst epithelial cells, differentiated cells (on the luminal surface) were identified by CK20 expression while proliferative cells were marked by Ki67 expression. Amongst leukocytes, myeloid (monocyte and others) cells were marked by CD11b, while proliferative leukocytes were marked by Ki67.