Figure 10.

Lack of mTORC1 inhibited Drp1 activation. Macrophages were incubated with or without AHs for 6 hours. (A) Immunofluorescence staining of the co-localization of Drp1 (green) and mTOR (red). Scale bar: 10 μm. Similar results were obtained from 5 independent experiments; a representative result is shown. (B) Immunoprecipitation (IP) of Drp1 and Raptor, and Western blot analysis (IB) of Drp1 and Raptor protein level. (C) Western blot analysis of protein levels of p-Drp1 (Ser616), total Drp1, p-p70S6K, and total p70S6K and quantification; β-actin was an internal control. Similar results were obtained in 3 independent experiments. Horizontal lines indicate the mean value and error bars represent the SEM. *P < .05. DAPI, 4′,6-diamidino-2-phenylindole; IB, immunoblotting; IP, Immunoprecipitates.