Figure 3.

Macrophage-specific Raptor deletion exacerbates inflammation in mice after feeding a FPC diet. Raptor^flox and Raptor^MacKO mice were fed the NCD or FPC diet for 24 weeks. (A) Liver weight, adipose tissue weight, and body weight of mice were measured, and (B) intraperitoneal glucose tolerance test was performed and glucose levels were measured. (C) Liver sections from all mice were stained with H&E, CD68, and Mac-3 antibodies for macrophages. Scale bars: 100 μm (upper panel); 20 μm (middle panel); and 50 μm (lower panel). (D) Quantification of the number of CD68-positive cells per field (upper panel) and morphometric analysis of Mac-3–stained sections (lower panel). (E) Plasma ALT and AST levels in mice. (F) Hepatic mRNA levels of IL1β, TNFα, MCP-1, and iNOS were determined by quantitative polymerase chain reaction analysis. (G) Oil-red O (ORO) staining of liver showing hepatic lipid accumulation. Scale bar: 100 μm. (H) Hepatic triglycerides (TG) and cholesterol (CHO) content. n = 5-6 animals per group; each point represents an individual animal, horizontal lines indicate the mean value, and error bars represent the SEM. *P < .05. DAPI, 4′,6-diamidino-2-phenylindole.