Figure 4.

Macrophage-specific Raptor deletion exacerbates inflammation in mice after feeding a MCD diet. Raptor^flox and Raptor^MacKO mice were fed an NCD or MCD diet for 4 weeks. (A) Liver weight and body weight were measured. (B) Representative images of H&E staining, immunofluorescence of CD68, and immunohistochemistry of Mac-3 in liver sections from all mice. Scale bars: 100 μm (upper panel); 20 μm (middle panel); and 50 μm (lower panel). (C) Quantification of the number of CD68-positive cells per field and Mac-3–positive area. (D) Plasma ALT and AST levels in mice. (E) Hepatic mRNA levels of IL1β, TNFα, MCP-1, and iNOS were determined by quantitative polymerase chain reaction analysis. (F) Oil-red O (ORO) staining of liver showing hepatic lipid accumulation. Scale bar: 100 μm. (G) Hepatic triglycerides (TG) and cholesterol (CHO) content. n = 6-7 animals per group. Each point represents an individual animal, horizontal lines indicate the mean value, and error bars represent the SEM. *P < .05. DAPI, 4′,6-diamidino-2-phenylindole.