Figure 8.

Macrophages from Raptor^MacKOmice showed phagolysosome dysfunction. (A) LysoTracker (red) was used to label lysosomes after macrophages were treated with SE-labeled AH (green) for 6 hours. Representative LysoTracker, succinimidyl easter (SE), and merge images are shown. Scale bar: 10 μm. Similar results were obtained in 5 independent experiments. (B) AHs were labeled with SE (green), and lysosomes were labeled with LysoTracker (red). Live images of AH digestion (arrows) in lysosomes were taken every 15 minutes for 6 hours. Also see Supplementary Movies 1 and 2. (C) The quantification of the size of engulfed AHs by macrophages in the Supplementary Movies 1 and 2. (B and C) Similar results were obtained in 3 independent experiments; a representative result is shown. (D) LysoTracker (red) was used to label lysosomes after macrophages were treated with succinimidyl ester–labeled apoptotic Jurkat cells (AC) (green) for 6 hours. Representative LysoTracker, AC, and merge images are shown. Scale bar: 10 μm. Similar results were obtained in 3 independent experiments; a representative result is shown.