Figure 5.

The effect of celastrol on the expression of NF-_KB. (a) Relative NF-_KB mRNA expression. (b) Relative NF-_KB protein expression in the cytoplasm and nucleus. Control: H9c2 cells are cultured under normoxic condition for 12 h; celastrol: H9c2 cells were treated with 50 nM celastrol for 1 h followed by culture under normoxic condition for 12 h. H/R: H9c2 cells were subjected to hypoxia for 8 h followed by reoxygenation for 4 h; H/R+ celastrol: H9c2 cells were treated with 50 nM celastrol for 1 h followed by hypoxia for 8 h and reoxygenation for 4 h. *represents P<0.05 vs. control group; # represents P<0.05 vs. H/R group