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Complementation of Saccharomyces cerevisiae nfs1:: KANMX4 knockout haploids with Cryptosporidium parvum IscS and random spore analysis. Top panel, spores‐derived colonies were streaked onto YEPD, 200 μg/ml G418 plates to select for clones that lack the NFS1 yeast gene. Bottom panel, spores‐derived were streaked onto SC‐Leu plates, in parallel to select for strains that had taken in the plasmid pBEVY‐L/C. parvum IscS. All colonies were checked for haploidy (see Fig. S7).
