Table 1.

Sequences of primers and probes targeting COI and 18S genes. Amplicon lengths were of 396 bp for COI and 80 bp for 18S

	COI gene	18S gene
Forward primers	5′‐CGACCCTCAGGCATAACCTT‐3′	5′‐AATCAGCGTGTCTTCCCTGG‐3′
Reverse primers	5′‐AGGCTCGAGTATCAACGTCTA‐3′	5′‐CAATTGCAAGCCCCAATCCC‐3′
Probes	5′‐HEX‐GGAAGCCTTTGGAACTTTAGG‐BHQ‐3′	5′‐FAM‐GTAACCCGCTGAACCTCCTT‐BHQ‐3′