Table 2.
Results of multiplex Aethina tumida PCR system application to 49 different insect DNA
| Individual | Species with GBOLD accession number where relevant | Origin | Development stage | Atum Cq | 18S Cq |
|---|---|---|---|---|---|
| 1 | Aethina tumida | Italy | Adult | 28.7 | 28.6 |
| 2 | Aethina tumida | Italy | Larvae | 30.9 | 26.4 |
| 3 | Aethina tumida | Italy | Larvae | 32.5 | 26.1 |
| 4 | Aethina tumida | Italy (Calabria) | Adult | 21 | 22.8 |
| 5 | Aethina tumida | Italy (Calabria) | Adult | 20.2 | 22.1 |
| 6 | Aethina tumida | United Kingdom (breeding from an US strain) | Larvae | 35.2 | 28.2 |
| 7 | Aethina tumida | United Kingdom (breeding from an US strain) | Adult | 30.3 | 26.2 |
| 8 | Aethina tumida | Mexico | Adult | 18.8 | 21.4 |
| 9 | Aethina tumida | South Africa | Adult | 27.9 | 34.1 |
| 10 | Aethina tumida | South Africa | Adult | 28 | 33.4 |
| 11 | Aethina tumida | South Africa | Larvae | 28 | 31.5 |
| 12 | Aethina tumida | South Africa | Larvae | 22.4 | 30 |
| 13 | Harmonia axyridis | Switzerland | Adult | ND | 16.1 |
| 14 | Harmonia axyridis | Switzerland | Adult | ND | 23.5 |
| 15 | Muscidae | Switzerland | Adult | ND | 42.1 |
| 16 | Forficula auricularia | Switzerland | Adult | ND | 38.5 |
| 17 | Leptoglossus occidentalis | Switzerland | Adult | ND | 37.1 |
| 18 | Galleria mellonella | Switzerland | Larvae | ND | 21.4 |
| 19 | Galleria mellonella | Switzerland | Larvae | ND | 22.8 |
| 20 | Lepidoptera | Switzerland | Larvae | ND | 32.7 |
| 21 | Varroa destructor | Switzerland | Adult | ND | 31 |
| 22 | Meligethes viridescens | Switzerland | Adult | ND | 35 |
| 23 |
Cychramus luteus (Nitidulidae)
ZFMK‐TIS‐2504554 |
Germany | Adult | ND | 34.9 |
| 24 |
Cychramus luteus (Nitidulidae)
ZFMK‐TIS‐2503863 |
Germany | Adult | ND | 33.3 |
| 25 |
Cychramus luteus (Nitidulidae)
ZFMK‐TIS‐2506747 |
Italy | Adult | ND | 34.7 |
| 26 |
Epuraea aestiva (Nitidulidae)
ZFMK‐TIS‐13931 |
Germany | Adult | ND | 24.3 |
| 27 |
Epuraea aestiva (Nitidulidae)
ZFMK‐TIS‐2504534 |
Germany | Adult | ND | 29.4 |
| 28 |
Epuraea aestiva (Nitidulidae)
ZFMK‐TIS‐2504535 |
Germany | Adult | ND | 28.4 |
| 29 |
Glischrochilus hortensis (Nitidulidae)
ZFMK‐TIS‐2522755 |
Germany | Adult | ND | 30.6 |
| 30 |
Glischrochilus hortensis (Nitidulidae)
ZFMK‐TIS‐11274 |
Germany | Adult | ND | 37.5 |
| 31 |
Glischrochilus hortensis (Nitidulidae)
ZFMK‐TIS‐11650 |
Germany | Adult | ND | 29.5 |
| 32 |
Glischrochilus quadriguttatus (Nitidulidae)
ZFMK‐TIS‐2515238 |
Germany | Adult | ND | 27.9 |
| 33 |
Glischrochilus quadriguttatus (Nitidulidae)
ZFMK‐TIS‐2511771 |
Germany | Adult | ND | 31.4 |
| 34 |
Glischrochilus quadriguttatus (Nitidulidae)
ZFMK‐TIS‐2521080 |
Germany | Adult | ND | 25.4 |
| 35 | Glischrochilus quadriguttatus (Nitidulidae) | Switzerland | Adult | ND | 35.2 |
| 36 | Glischrochilus quadriguttatus (Nitidulidae) | Switzerland | Adult | ND | 34.9 |
| 37 | Glischrochilus quadriguttatus (Nitidulidae) | Switzerland | Adult | ND | 30.9 |
| 38 |
Carpophilus sexpustulatus (Nitidulidae)
ZFMK‐TIS‐2521034 |
Germany | Adult | ND | 29.1 |
| 39 |
Carpophilus sexpustulatus (Nitidulidae)
ZFMK‐TIS‐2521033 |
Germany | Adult | ND | 26.4 |
| 40 |
Carpophilus sexpustulatus (Nitidulidae)
ZFMK‐TIS‐2527002 |
Germany | Adult | ND | 29.3 |
| 41 |
Carpophilus sp. (Nitidulidae)
ZFMK‐TIS‐2580556 |
Slovenia | Adult | ND | 27 |
| 42 | Soronia grisea (Nitidulidae) | Switzerland | Adult | ND | 35.7 |
| 43 | Soronia grisea (Nitidulidae) | Switzerland | Adult | ND | 38.2 |
| 44 | Soronia grisea (Nitidulidae) | Switzerland | Adult | ND | 29.9 |
| 45 | Trichodes alvearius | Switzerland | Adult | ND | 28.6 |
| 46 | Hoplia philanthus (Scarabaeidae) | Switzerland | Adult | ND | 17.7 |
| 47 | Hoplia philanthus (Scarabaeidae) | Switzerland | Adult | ND | 18.4 |
| 48 | Hoplia philanthus (Scarabaeidae) | Switzerland | Adult | ND | 19.3 |
| 49 | Hoplia philanthus (Scarabaeidae) | Switzerland | Adult | ND | 19.9 |
Note: ND, non detected.
Some DNA from insects (individuals 23–34 and 38–41) were obtained from BOLD Germany, details can be found at https://doi.org/10.5883/DS-AETHINA