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Electron transfer dissociation (ETD) mass spectra of (top) [AAAACAK + 2H]2+ and (bottom) [AAACAAK + 2H]2+. ETD fragment ions are annotated. Neutral loss of an SH• radical is observed for the z3 • fragment from AAAACAK and for the z4 • ion from AAACAAK, ie, from z •‐ions with the Cys residue at the cleavage site
