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. 2008 Aug 22;18(11):842–850. doi: 10.1093/glycob/cwn079

Fig. 3.

Fig. 3

Quantitative analysis confirms the increase of Gal-1 expression in the gastrocnemius (GA) and diaphragm (DIA) muscles during degeneration. (A and C) Coomassie-blue stained gels containing samples of GA and DIA of C57BL/6 (C57: 4-week-old mice) and mdx (3- to 6-week-old mice). (B and D) Equivalent immunoblots probed with anti-Gal-1 (and anti-desmin as constitutive control) were quantified by band densitometry. (E) Immunoblot with crescent amounts of hrGal-1 (18–250 ng/lane) used to build a standard curve to estimate Gal-1 content. In mdx GA (B), the expression of Gal-1 reaches a peak at 4 weeks (*P = 0.0002 when compared to age-matched C57) and decreases in the fifth and the sixth weeks (#P = 0.0025), resembling the degeneration curve observed in this period. In mdx DIA, no statistical significance was observed in Gal-1 expression between weeks, only in relation to the C57 mice (*P = 0.0002 and P = 0.0003, for the fourth and sixth weeks, respectively).