Fig. 6.

BaCl₂ injection induces muscle injury and increased Gal-1 expression in C57BL/6 mice. (A) Coomassie blue-stained gel of equal quantities of GA samples of C57BL/6 (C57) following injection with either NaCl (N) or BaCl₂ (B) at the indicated times (1, 3, and 7 days after the injection). (B) Immunoblot analysis of Gal-1 expression in either NaCl-treated (N) or BaCl₂-treated (B) animals. The numbers below the bands indicate the relative quantity of Gal-1 expression in the samples and are representative of three independent experiments. The intensities of the bands were calculated using the 1.37v Image-J software (NIH, Bethesda, MD). Gal-1 levels increased during degeneration starting from day 3, with the higher expression at day 7.