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. 2018 Nov 29;9:2789. doi: 10.3389/fimmu.2018.02789

Figure 1.

Figure 1

Splenic T and B cells form IMPDH filaments in vivo. (A) Representative image of a lymphoid follicle in an FFPE spleen section from an adult healthy B6 mouse stained for IMPDH (green) and T cell marker CD3 (red). Widespread IMPDH filament formation was detected throughout the spleen, including within the T cell zone (“T”), germinal centers (“GC”), other cells within the follicle, and even in the red pulp (“R”). (B) Representative image of the T cell zone showing many IMPDH filaments within T cells. (C) Representative image of a germinal center showing prominent IMPDH filaments in both CD3+ and CD3− cells (highlighted by yellow circles). (D) Representative images of two different mice, a 7-months-old female and a 12-months-old male. Images were captured using the same objective as (B,C) but were enlarged 2× to more clearly show IMPDH filaments within T cells. White arrows: ring structure. (E) Lymphoid follicle from an adult healthy B6 mouse spleen stained for IMPDH (green) and B cell marker CD19 (red). IMPDH filaments were detected in GC B cells, follicular (“F”) B cells, and marginal zone (“MZ”) B cells. (F) Representative image of a germinal center depicting numerous IMPDH filaments within B cells. (G) Representative image illustrating IMPDH filament formation in follicular (“F”) and marginal zone (“MZ”) B cells. (H) Representative images of two different mice, a 5-months-old male and a 12-months-old female. Images were captured using the same objective as (F,G) but were enlarged 2× to more clearly show IMPDH filaments within B cells. White arrows: ring structure. Counterstain for all panels: DAPI (blue). Scale bars: 50 μm (A,E), 10 μm (B,C,F,G), 5 μm (D,H).