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. 2018 Oct 15;210(4):1369–1381. doi: 10.1534/genetics.118.301551

Figure 2.

Figure 2

UNC-7 and UNC-9 is partially required in premotor interneurons and motor neurons, respectively, during sleep. (A) Total time in sleep bouts for unc-7(e5lf) animals in L4 to adult lethargus rescued with myo-3 promoter, aex-3 promoter, glr-1 promoter, and rig-3 promoter driven unc-7 and their corresponding promoter driven GFP as controls. Colors represent three independent extrachromosomal arrays tested for each condition (strain information in Table S2). (B) Total time in sleep bouts for unc-9(e101lf) animals in L4 to adult lethargus rescued with myo-3 promoter, aex-3 promoter, glr-1 promoter, and unc-4 promoter driven unc-9 and their corresponding promoter driven GFP as controls. Colors represent two or three independent extrachromosomal arrays tested for each condition (strain information in Table S2). (C) Percent of animals with anachronistic sleep in wild-type animals, animals overexpressing hsp::gfp ([control]), hsp::unc-7, hsp::unc-9, and both hsp::unc-7 and hsp::unc-9. Data from three independent extrachromosomal arrays were pooled for each genotype. At least three independent trials were tested. n = 10 per genotype for each trial. * P < 0.05, ** P < 0.01, *** P < 0.001. All error bars represent standard error of the mean.