EGL-4 PKG is downstream of NCA channels during L4/A lethargus. (A) Total time in sleep bouts for wild-type, unc-79(ec1lf), egl-4(n479lf), unc-79(ec1lf); egl-4(n479lf), egl-4(ad450gf), and unc-79(ec1lf); egl-4(ad450gf). (B) Response latency of wild-type, unc-79(ec1lf), egl-4(n479lf), and unc-79(ec1lf); egl-4(n479lf) animals to blue light stimulation during sleep bouts (left panel) and motion bouts (right panel). (C) Response latency of wild-type, unc-79(ec1lf), egl-4(ad450gf), and unc-79(ec1lf); egl-4(ad450gf) animals to blue light stimulation during sleep bouts (left panel) and motion bouts (right panel). All sleep bouts and motions bouts were assessed during L4 to adult lethargus. * P < 0.05, ** P < 0.01, *** P < 0.001. All error bars represent standard error of the mean.