Figure 4.

Phenotypic analysis of prp4ka and sac3a mutants. (A and B) The sac3a mutants appear largely normal by the measured criteria: transition to flowering (bolting), final height of adult plant, seed weight, and branch (stem) number. The prp4ka mutants feature delayed flowering, lowered seed set, reduced branching, a tall final stature, and somewhat flat, darker green rosettes [numerical values in Figure S1A (prp4ka and sac3a experiment) and Figure S1B (prp4kb experiment)]. (B) Tall stature is not visible in the photograph, which shows age-matched, wild-type (WT) and mutant plants, but is apparent in fully grown prp4ka plants (Figure S1A). (C) Complementation of the prp4ka mutants with a 35Spro-PRP4KA transgene restores a normal phenotype. Particularly visible in the age-matched samples shown here is the late transition to flowering and somewhat flat, darker green rosettes in the prp4ka mutant (left) compared to the complemented lines (right), which also have normal branching patterns (Figure S1B).