Table 2. High- and low-frequency amino acids in Ydj1p- and Ras-based data sets.

Position	Reporter	Above CILa	Within CIL	Below CIL
x1	Ydj1p	A T V D G S	N R E I K	F H Q W Y L M P; C (absent)
	Ras	A T V I L	N R C M S P	F H Q W Y D E G K
x2	Ydj1p	V A G H N Q S Y	T I L M P	K R W (all absent); D E C F
	Ras	V I L M	T C F	K R W G H N P S (all absent); D E A Q Y
x3	Ydj1p	Q S G H I N V	T A D M W	K P (all absent); E R Y C F L
	Ras	Q S A C M	T F I L N V	K P R W (all absent); E Y D G H

^aThe number of instances that each amino acid was observed at a particular position within the population of sequences was determined. For Ydj1-based sequences (n = 153), the number of occurrences for each amino acid was normalized to adjust for codon bias (i.e., there are more Leu than Met codons). For Ras2-based sequences (n = 369), normalization was not needed due to study design. An SD and 95% confidence interval level (CIL) was determined for each population at each position that was used to determine amino acids above or below the interval. Amino acids that are in both the Ydj1- and Ras-based data sets for an indicated frequency group are bold.