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. 2018 Oct 19;210(4):1527–1542. doi: 10.1534/genetics.118.301578

Figure 7.

Figure 7

Quantitative real-time PCR of candidate genes in the parental strains. (A) Expression of nine candidate genes in isolated pancreatic islets is shown relative to C3H. Genes were selected based on differential expression between C3H and NZO in the microarray analysis, and location in C3H polymorphic regions. Significance is indicated for genes with similar expression patterns between NZO and 129P2, but differing from C3H. (B) Expression of Pop4 and Atp4a in liver tissue. Both tissues were collected at the age of 6 weeks and Tbp was used as endogenous control. Data represent mean values ± SEM from five to eight mice. Statistical differences between strains were calculated by one-way ANOVA followed by post hoc Bonferroni test; ** P < 0.01 and *** P < 0.001 (in (A): by comparison to C3H). NZO, New Zealand Obese.