Figure 8.

Quantitative real-time PCR of Pop4 and Atp4a in the recombinant congenic strains. Relative expression in C3H/C3H and NZO/NZO allele carriers for chromosome 7 in isolated pancreatic collected at 6 weeks of age (A) and liver tissue collected at 17 weeks of age (B). Tbp was used as endogenous control. Data represent mean values ± SEM from five to eight mice. Statistical differences between the genotypes were calculated by Student’s t-test, two-tailed, unpaired; * P < 0.05, ** P < 0.01, and *** P < 0.001. NZO, New Zealand Obese; RCS, recombinant congenic strain.