Skip to main content
. 2018 Dec 3;10:6599–6609. doi: 10.2147/CMAR.S187973

Figure 5.

Figure 5

lncRNA 1308 is a target of miR-124 and miRNA that represses protein levels of ADAM 15

Notes: (A and B) Schematic representation of pMIR firefly luciferase reporter construction. (A) A 525-bp lncRNA 1308 sequence was cloned and constructed in pMIR firefly luciferase reporter. (B) 274 bp of ADAM 15 3′-UTR sequence was cloned and constructed in pMIR firefly luciferase reporter. (C and D) Analysis of luciferase activity. HEK 293T cells were co-transfected with pMIR firefly luciferase reporter plasmids and pTK-Renilla luciferase plasmids, together with negative control and miR-124 mimics. After 36 h, firefly luciferase activity was measured and normalized by Renilla luciferase activity. (E) Effects of miR-124 mimics on the endogenous ADAM 15 protein levels. XWLC and GLC cells were cotransfected with miR-124 mimics or NC. Forty-eight hours after transfection, the cells were isolated, and the expression of ADAM 15 was analyzed by Western blotting. Results were represented as mean ± SD. **P<0.01.

Abbreviations: ADAM15, a disintegrin and a metalloproteinase 15; GLC, Gejiu lung cancer; lncRNA 1308, long non-coding RNA 1308; miRNA, microRNA; NC, negative control oligonucleotides; ns, not significant; XWLC, Xuanwei lung cancer.