Figure 6.

Combinatorial therapy significantly alters the number and phenotype of microglia/macrophages present in the spinal cord in EAE. At day 21 post-EAE induction, flow cytometry was performed on whole spinal cord lysates from control or treated mice. CD11b was used to identify the microglia/macrophage population. CD11b⁺CD86⁺ cells were considered of a pro-inflammatory nature and CD11b⁺CD206⁺ cells were considered anti-inflammatory (A). Total CD11b⁺ cells (B) were plotted as well as percentage of CD11b⁺ cells that were CD86⁺ (C) or CD206⁺ (D). Data are mean ± SEM. n = 3–4, *p < 0.05, **p < 0.01 compared to control. ^#p < 0.05 compared to benztropine.