Fig. 1.

Nutrient availability affects histone acetylation and cellular acetyl-CoA levels. A, Schematic outline of the experimental setup. Yeast cells were grown in rich medium and harvested at the indicated growth phases. Stationary cells were given 0%, 2% or 10% (w/v) of glucose and harvested after an additional 24 h of growth. B, Histone acetylation declines as yeast cells progress to stationary phase, but is partially restored after glucose addition. Immunoblot analysis of H4 acetylation, bulk H4 expression, and actin (loading control) at indicated time points. C, Intracellular acetyl-CoA levels changes with nutrient availability. Metabolites were extracted from whole yeast cells at the indicated growth phases and acetyl-CoA levels were measured by MS. Data were normalized to the average value at 6 h. Normalized data are shown as log10. Mean ± S.D. is shown as lines. 6 h, 100%; 3 days, 2%; 8 days, 1%, 9 days + 0% glucose, 1%; 9 days + 2% glucose, 5%; 9 days + 10% glucose, 30%. ****, p < 0.0001; *, p < 0.05; n = 6.