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. 2018 Aug 27;17(12):2309–2323. doi: 10.1074/mcp.RA118.000982

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© 2018 Varland et al.

Published by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Fig. 2. — Metabolic effect on Nt-acetylation in yeast. A, Total distribution of annotated and alternative N termini identified and overall acetylation trend following prolonged starvation. Free N termini refers to N termini that cannot be acetylated (MP- and P- starting). (B-C) Differences in Nt-acetylation of affected N termini upon entry into stationary phase and following glucose addition. k-means clustering of affected N termini (104) revealed two distinct clusters (p = 0.000167). B, Cluster 1 is enriched for annotated N termini (blue) that are more Nt-acetylated in stationary phase and experiences a reduction of Nt-acetylation upon glucose addition. C, Cluster 2 is enriched for alternative N termini (red) and show an acetylation trend that is opposite of cluster 1.