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. 2018 Aug 27;17(12):2309–2323. doi: 10.1074/mcp.RA118.000982

Fig. 6.

Fig. 6.

Immunoblot analysis of differentially Nt-acetylated proteins under different metabolic conditions and in presence/absence of the cognate NAT. Wild-type, naa10Δ and naa30Δ yeast strains endogenously expressing a unique TAP-tagged protein were grown in rich medium and harvested at indicated time points. Whole cell extracts were analyzed by immunoblotting using anti-TAP and anti-actin (loading control). Two individual deletion strains per candidate protein were used. The levels of TAP-tagged candidate proteins relative to actin at each time point were quantified by densitometry analysis and normalized to the wild-type sample within the same metabolic condition.