Table I. Selected N-termini that are subject to differential Nt-acetylation upon entry into stationary phase.

Standard name	Systematic name	N'term	Start	N-terminal acetylation in percentage						Cluster
				6 hrs	3 days	8 days	Day 9a
							0%	2%	10%
Annotated N-termini
Atg8	YBL078C	MKSTF	1	26	28	65	63	44	38	ø
Atg22	YCL038C	SYGTI	2	-	60	-	55	58	69	-
Pbp1	YGR178C	MKGNF	1	72	93	100	100	94	92	5
Pcl8	YPL219W	ANDQD	2	74	92	-	100	86	-	-
Rpl7a	YGL076C	AAEKI	2	12	44	43	47	50	-	5
Rsa3	YLR221C	SAGDI	2	42	72	-	70	71	-	-
Smy2	YBR172C	MIAPD	1	34	59	100	90	53	57	ø
Tom70	YNL121C	MKSFI	1	18	47	46	50	56	56	ø
Tps1	YBR126C	TTDNA	2	34	44	46	48	49	56	ø
Tps2	YDR074W	TTTAQ	2	79	86	90	88	89	91	2
Alternative N-termini
Eis1	YMR031C	METTS	164	48	38	13	4	16	-
Fbp1	YLR377C	MEQAG	300	-	6	34	21	-	-
Pgi1	YBR196C	MESNG	366	-	86	39	21	41	82

^aStationary cells (day 8) were given 2% or 10% (w/v) glucose and harvested after additional 24 hours of growth. Hyphen (-) indicates that the N-terminus was not detected in the particular sample. Ø denotes samples that were detected and quantified by shotgun proteomics, but whose expression level was not significantly affected between the different conditions. In general, proteins in cluster 2 showed higher expression in stationary phase whereas proteins in cluster 5 were less expressed in stationary phase.