Large-scale and high-precision CCS measurements.
A, Pearson correlation of peptide ion mobilities in two replicate injections of a HeLa digest (100 ms TIMS ramps). B, Relative deviations of TIMSCCS values of all individual peptides from their mean of quadruplicate LC-MS runs (n = 144,363; 1,186 out of range). C, Pearson correlation of measured TIMSCCS values in two injections of a HeLa digest with different TIMS ramp times (100 and 200 ms TIMS ramps). D, Density distribution of 129,110 TIMSCCS values from human tryptic peptide ions as a function of m/z. The main populations are annotated with their respective charge states.