Changes in cell surface glycosylation following differentiation of primary and immortalized healthy human myotubes. Healthy primary (HSMC-1, -2, -3, -4) and immortalized (LHCN-M2) human myoblasts (d0, solid bars) and myotubes (d5, hatched bars) were characterized with a panel of 13 lectins. Increased binding of four O-glycan-specific lectins as well as NPA and MAA-II (high-mannose N-glycan and sialic acid-specific, respectively) was observed for both immortalized healthy human muscle cells and four primary healthy human muscle cell preparations. This figure is available in black and white in print and in color at Glycobiology online.