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. 2017 Jan 17;30(2):113–118. doi: 10.1093/protein/gzw070

Fig. 2.

Fig. 2

Binding and inhibition tests of 1F8 mutants. (A) In ELISA, 10 nM Fab 1F8 or its mutants was incubated with cdMMP-14 immobilized on the plates and detected by anti-Fab-HRP. Signal was recorded upon reaction with TMB and stopped by addition of 1 M H2SO4. (B) In FRET inhibition tests, activities of 1 nM cdMMP-14 were measured with 1 µM of quenched-fluorescent peptide substrate at the presence of 500 nM Fab 1F8 or its mutants.