Table 1.
Tissue | THAI #4 (T4) | THAI #23 (T4) | THAI #18 (T4) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Eu (RLU/mg Protein) | Hyper (RLU/mg Protein) | Fold (Means ± SEM) | P Value | Eu (RLU/mg Protein) | Hyper (RLU/mg Protein) | Fold (Means ± SEM) | P Value | Eu (RLU/mg Protein) | Hyper (RLU/mg Protein) | Fold (Means ± SEM) | P Value | |
Pit | 0.337 | 0.791 | 2.3 ± 0.16 | 0.0001a | 1.932 | 16.926 | 8.8 ± 0.62 | 0.0001a | 0.066 | 0.54 | 8.2 ± 0.36 | 0.0001a |
MBH | 0.206 | 0.368 | 1.8 ± 0.20 | 0.013a | 0.142 | 0.24 | 1.7 ± 0.29 | 0.103 | 0.372 | 0.602 | 2.2 ± 0.25 | 0.005a |
HC | 0.06 | 0.116 | 1.9 ± 0.03 | 0.0001a | 0.035 | 0.047 | 1.5 ± 0.35 | 0.201 | 2.308 | 3.756 | 2.1 ± 0.25 | 0.013a |
HT | 0.377 | 0.488 | 1.3 ± 0.08 | 0.031a | 0.65 | 0.78 | 1.2 ± 0.11 | 0.176 | 0.755 | 1.589 | 2.8 ± 0.35 | 0.004a |
CTX | 0.194 | 0.359 | 1.9 ± 0.30 | 0.035a | 0.179 | 0.253 | 1.4 ± 0.13 | 0.017a | 1.617 | 2.586 | 1.9 ± 1.18 | 0.005a |
CER | 0.938 | 2.162 | 2.3 ± 0.41 | 0.019a | 0.012 | 0.023 | 1.9 ± 0.28 | 0.045a | 0.045 | 0.063 | 1.8 ± 0.31 | 0.073 |
Liver | 0.002 | 0.005 | 1.9 ± 0.14 | 0.0007a | 0.001 | 0.003 | 2.9 ± 0.86 | 0.064 | No detectable expression | |||
Heart | 0.004 | 0.03 | 8.5 ± 0.31 | 0.0001a | 0.001 | 0.001 | 1.0 ± 0.25 | – | No detectable expression | |||
BAT | 0.028 | 1.82 | 64.6 ± 8.1 | 0.0002a | 0.005 | 0.038 | 7.2 ± 1.54 | 0.007a | No detectable expression | |||
Bone | 0.031 | 0.066 | 2.2 ± 0.18 | 0.0015a | 0.01 | 0.011 | 0.7 ± 0.11 | 0.057 | No detectable expression | |||
Muscle | 0.025 | 0.276 | 11.2 ± 5.7 | 0.0041a | 0.004 | 0.003 | 0.7 ± 0.18 | 0.115 | No detectable expression | |||
MSG | 0.052 | 0.399 | 7.6 ± 2.60 | 0.044a | 0.004 | 0.011 | 2.7 ± 0.77 | 0.072 | Not determined | |||
Thyroid | 0.115 | 0.176 | 1.5 ± 0.07 | 0.002a | 0.026 | 0.03 | 0.8 ± 0.18 | 0.734 | Not determined | |||
Testicle | 4876 | 4086 | 0.8 ± 0.06 | 0.108 | 2909 | 2509 | 0.9 ± 0.11 | 0.253 | Not determined | |||
Intestine | 4.571 | 9.48 | 2.1 ± 0.30 | 0.012a | 0.041 | 0.212 | 5.2 ± 1.6 | 0.029a | Not determined | |||
Skin | 0.696 | 2.798 | 4.0 ± 0.76 | 0.001a | 2.358 | 1.878 | 0.8 ± 0.17 | 0.544 | Not determined |
Euthyroid animals were treated with L-T4 (IP 5 μg/d per animal for 3 days). Luciferase activity was assessed in tissue samples with luciferase assay and expressed as RLU per milligram protein. Luciferase activity of corresponding tissues of TH vs vehicle-treated animals was performed by Student t test (n ≥ 4).
Abbreviations: CER, cerebellum; CTX, cerebral cortex; Eu, euthyroid; HC, hippocampus; HT, hypothalamus; Hyper, hyperthyroid; MBH, mediobasal hypothalamus; MSG, mandibular salivary gland; Pit, pituitary.
P < 0.05.